PMID:8830707

Citation	Moriyama, R, Hattori, A, Miyata, S, Kudoh, S and Makino, S (1996) A gene (sleB) encoding a spore cortex-lytic enzyme from Bacillus subtilis and response of the enzyme to L-alanine-mediated germination. J. Bacteriol. 178:6059-63
Abstract	The Bacillus subtilis sleB gene, which codes for the enzyme homologous to the germination-specific amidase from Bacillus cereus, was cloned and its nucleotide sequence was determined. Sequence analysis showed that it had an open reading frame of 918 bp, coding for a polypeptide of 305 amino acids with a putative signal sequence of 29 residues. Enzyme activity was not found in germination exudate of B. subtilis spores, which differs from the case of B. cereus enzyme. A B. subtilis mutant with an insertionally inactivated sleB gene revealed normal behavior in growth and sporulation. However, the sleB mutant was unable to complete germination mediated by L-alanine.
Links	PubMed PMC178467
Keywords	Alanine/pharmacology; Amidohydrolases/genetics; Amino Acid Sequence; Bacillus subtilis/cytology; Bacillus subtilis/drug effects; Bacillus subtilis/physiology; Bacterial Proteins/genetics; Bacteriolysis; Base Sequence; Cloning, Molecular; Genes, Bacterial; Molecular Sequence Data; Mutagenesis, Insertional; Restriction Mapping; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Spores, Bacterial/cytology; Spores, Bacterial/drug effects; Stereoisomerism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BACSU:SLEB		GO:0009847: spore germination	ECO:0000315:		P		Table 1. The above results indicate that the B. subtilis sleB gene is certainly expressed in the spore and that product of the gene responds to, at least, L-alanine-triggered germination.	complete CACAO 2778

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