PMID:8820608

Citation	Nakagawa, H, Ohmiya, K and Hattori, T (1996) A rice bZIP protein, designated OSBZ8, is rapidly induced by abscisic acid. Plant J. 9:217-27
Abstract	A cDNA that encoded a bZIP protein, designated OSBZ8, was isolated from a rice embryo cDNA library by use of degenerate oligonucleotide probes that corresponded to the amino acid sequences conserved among the basic regions of plant G-box-binding factor-type bZIP proteins (GBF). OSBZ8 was shown to have structural features typical of the GBF-type bZIP proteins and to bind to G-box and G-box-like sequences that include ABA-responsive elements (ABREs) which have been functionally identified in the promoters of ABA-inducible genes, such as Em, Osem and Rab16. The accumulation of OSBZ8 mRNA was induced by treatment with ABA of imbibed mature rice embryos, of young plant tissues and of suspension-cultured cells. The accumulation of OSBZ8 mRNA in response to ABA preceded that of Osem and Rab16A mRNAs and was not inhibited by an inhibitor of protein synthesis, cycloheximide. By contrast, the induction of Osem and Rab16A was partially inhibited and almost completely inhibited, respectively, by cycloheximide. These results strongly suggest that OSBZ8 might be involved in the regulation of transcription by ABA in rice.
Links	PubMed
Keywords	Abscisic Acid/pharmacology; Amino Acid Sequence; Base Sequence; Basic-Leucine Zipper Transcription Factors; Blotting, Northern; Blotting, Southern; Cloning, Molecular; Cycloheximide/pharmacology; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; G-Box Binding Factors; Gene Expression Regulation, Plant; Leucine Zippers; Molecular Sequence Data; Oryza sativa/genetics; Plant Proteins/genetics; Plant Proteins/metabolism; Protein Binding; Protein Synthesis Inhibitors/pharmacology; Recombinant Fusion Proteins/metabolism; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Tissue Distribution; Transcription Factors
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ORYSA:Q40645		GO:0000976: transcription regulatory region sequence-specific DNA binding	ECO:0000314:		F		Figures 4b and 4c	complete CACAO 8190
ORYSA:Q40645	enables	GO:0000976: transcription regulatory region sequence-specific DNA binding	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete

References

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