PMID:8808622

Citation	Posas, F, Wurgler-Murphy, SM, Maeda, T, Witten, EA, Thai, TC and Saito, H (1996) Yeast HOG1 MAP kinase cascade is regulated by a multistep phosphorelay mechanism in the SLN1-YPD1-SSK1 "two-component" osmosensor. Cell 86:865-75
Abstract	An osmosensing mechanism in the budding yeast (Saccharomyces cerevisiae) involves both a two-component signal transducer (Sln1p, Ypd1p and Ssk1p) and a MAP kinase cascade (Ssk2p/Ssk22p, Pbs2p, and Hog1p). The transmembrane protein Sln1p contains an extracellular sensor domain and cytoplasmic histidine kinase and receiver domains, whereas the cytoplasmic protein Ssk1p contains a receiver domain. Ypd1p binds to both Sln1p and Ssk1p and mediates the multistep phosphotransfer reaction (phosphorelay). This phosphorelay system is initiated by the autophosphorylation of Sln1p at His576. This phosphate is then sequentially transferred to Sln1p-Asp-1144, then to Ypd1p-His64, and finally to Ssk1p-Asp554. We propose that the multistep phosphorelay mechanism is a universal signal transduction apparatus utilized both in prokaryotes and eukaryotes.
Links	PubMed
Keywords	Amino Acid Sequence; Aspartic Acid/metabolism; Binding Sites; Calcium-Calmodulin-Dependent Protein Kinases/metabolism; Cloning, Molecular; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Fungal Proteins/genetics; Fungal Proteins/metabolism; Genes, Fungal; Histidine/metabolism; Intracellular Signaling Peptides and Proteins; Mitogen-Activated Protein Kinases; Molecular Sequence Data; Phenotype; Phosphorylation; Protein Kinases; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins; Signal Transduction/physiology; Water-Electrolyte Balance/physiology
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