PMID:8706131

Citation	Bai, C, Sen, P, Hofmann, K, Ma, L, Goebl, M, Harper, JW and Elledge, SJ (1996) SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. Cell 86:263-74
Abstract	We have identified the yeast and human homologs of the SKP1 gene as a suppressor of cdc4 mutants and as a cyclin F-binding protein. Skp1p indirectly binds cyclin A/Cdk2 through Skp2p, and directly binds Skp2p, cyclin F, and Cdc4p through a novel structural motif called the F-box. SKP1 is required for ubiquitin-mediated proteolysis of Cin2p, Clb5p, and the Cdk inhibitor Sic1p, and provides a link between these molecules and the proteolysis machinery. A large number of proteins contain the F-box motif and are thereby implicated in the ubiquitin pathway. Different skp1 mutants arrest cells in either G1 or G2, suggesting a connection between regulation of proteolysis in different stages of the cycle.
Links	PubMed
Keywords	Amino Acid Sequence; Base Sequence; Binding Sites/physiology; Cell Cycle/genetics; Cell Cycle Proteins/chemistry; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Cell Cycle Proteins/physiology; Cyclins/chemistry; Cyclins/genetics; Cyclins/metabolism; F-Box Proteins; Fungal Proteins/physiology; G1 Phase/physiology; G2 Phase/physiology; Gene Dosage; Gene Expression/physiology; Humans; Ligases/genetics; Mitosis/physiology; Molecular Sequence Data; Molecular Structure; Mutation/physiology; Protein Structure, Tertiary; Proteins/metabolism; S Phase/physiology; S-Phase Kinase-Associated Proteins; Saccharomyces cerevisiae Proteins; Ubiquitin-Protein Ligase Complexes; Ubiquitin-Protein Ligases; Ubiquitins/physiology; Yeasts/genetics
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