PMID:8699248

Citation	Takahashi, T, Feldmeyer, D, Suzuki, N, Onodera, K, Cull-Candy, SG, Sakimura, K and Mishina, M (1996) Functional correlation of NMDA receptor epsilon subunits expression with the properties of single-channel and synaptic currents in the developing cerebellum. J. Neurosci. 16:4376-82
Abstract	NMDA receptor (NMDAR) subunits epsilon 1-epsilon 4 are expressed differentially with respect to brain region and ontogenic period, but their functional roles still are unclear. We have compared an epsilon 1 subunit-ablated mutant mouse with the wild-type to characterize the effect of epsilon subunit expression on NMDAR-mediated single-channel currents and synaptic currents of granule cells in cerebellar slices. Single-channel and Western blot analyses indicated that the epsilon 2 subunit disappeared gradually during the first postnatal month in both wild-type and mutant mice. Concomitantly, the voltage-dependent Mg2+ block of NMDAR-mediated EPSCs (NMDA-EPSCs) was decreased. Throughout the developmental period studied, postnatal day 7-24 (P7-P24), the decay time course of NMDA-EPSCs in epsilon 1 mutant (-/-) mice was slower than in wild-type mice. We suggest that the expression of the epsilon 3 subunit late in development is responsible for a reduction in the sensitivity of NMDA-EPSCs to block by extracellular Mg2+ and that receptors containing the epsilon 1 subunit determine the fast kinetics of the NMDA-EPSCs.
Links	PubMed
Keywords	Age Distribution; Animals; Animals, Newborn; Cerebellum/physiology; Magnesium/pharmacology; Mice; Mice, Mutant Strains; Patch-Clamp Techniques; Presynaptic Terminals/physiology; Receptors, N-Methyl-D-Aspartate/physiology
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