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PMID:8656286
| Citation |
Feng, G, Hannan, F, Reale, V, Hon, YY, Kousky, CT, Evans, PD and Hall, LM (1996) Cloning and functional characterization of a novel dopamine receptor from Drosophila melanogaster. J. Neurosci. 16:3925-33 |
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| Abstract |
A cDNA clone is described that encodes a novel G-protein-coupled dopamine receptor (DopR99B) expressed in Drosophila heads. The DopR99B receptor maps to 99B3-5, close to the position of the octopamine/tyramine receptor gene at 99A10-B1, suggesting that the two may be related through a gene duplication. Agonist stimulation of DopR99B receptors expressed in Xenopus oocytes increased intracellular Ca2+ levels monitored as changes in an endogenous inward Ca2+-dependent chloride current. In addition to initiating this intracellular Ca2+ signal, stimulation of DopR99B increased cAMP levels. The rank order of potency of agonists in stimulating the chloride current is: dopamine > norepinephrine > epinephrine > tyramine. Octopamine and 5-hydroxytryptamine are not active (< 100 microM). This pharmacological profile plus the second-messenger coupling pattern suggest that the DopR99B receptor is a D1-like dopamine receptor. However, the hydrophobic core region of the DopR99B receptor shows almost equal amino acid sequence identity (40-48%) with vertebrate serotonergic, alpha 1- and beta-adrenergic, and D1-like and D2-like dopaminergic receptors. Thus, this Drosophila receptor defines a novel structural class of dopamine receptors. Because DopR99B is the second dopamine receptor cloned from Drosophila, this work establishes dopamine receptor diversity in a system amenable to genetic dissection. |
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| Keywords |
Amino Acid Sequence; Animals; Base Sequence; Blotting, Northern; Calcium/metabolism; Chromosome Mapping; Chromosomes/genetics; Cloning, Molecular; Cyclic AMP/metabolism; DNA, Complementary/genetics; Drosophila melanogaster/genetics; Female; GTP-Binding Proteins/genetics; In Situ Hybridization; Molecular Sequence Data; Nervous System Physiological Phenomena; Oocytes/physiology; RNA, Messenger/analysis; Receptors, Dopamine/genetics; Second Messenger Systems/genetics; Sequence Analysis, DNA; Xenopus |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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