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PMID:8619862
| Citation |
Ziegler, I and Gütlich, M (1996) Tetrahydropterins interfere with the G protein pathway in Dictyostelium discoideum. Biochem. Biophys. Res. Commun. 221:368-73 |
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| Abstract |
The cellular slime mold Dictyostelium discoideum produces tetrahydrodictyopterin, the D-threo-isomer of tetrahydrobiopterin. During cyclic AMP coordinated aggregation, the G protein linked signalling pathway is involved in the regulation of the initial enzyme GTP cyclohydrolase I [M. Gütlich et al., Biochem. J. 314, 95-101 (1996)]. We now find that cyclic AMP stimulated binding of GTPgammaS to the membrane fraction is inhibited by tetrahydrodictyopterin and tetrahydrobiopterin. Inhibition was a function of GTPgammaS concentration and the analysis of the kinetic data pointed to a competitive type of inhibition. The inhibition of G protein activation was accompanied by a loss of adenylyl cyclase activation. Because the GTPgammaS- and G protein-dependent reduction of receptor affinity for cyclic AMP was also attenuated, we conclude that tetrahydrodictyopterin and tetrahydrobiopterin interfere with activation of G proteins by inhibiting GDP-GTP exchange. |
| Links |
PubMed Online version:10.1006/bbrc.1996.0602 |
| Keywords |
Adenylate Cyclase/metabolism; Animals; Biopterin/analogs & derivatives; Biopterin/metabolism; Cyclic AMP/metabolism; Dictyostelium/metabolism; Enzyme Activation; GTP-Binding Proteins/metabolism; Guanosine 5'-O-(3-Thiotriphosphate)/antagonists & inhibitors; Guanosine 5'-O-(3-Thiotriphosphate)/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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