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PMID:8573145
| Citation |
Nardese, V, Gütlich, M, Brambilla, A and Carbone, ML (1996) Disruption of the GTP-cyclohydrolase I gene in Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 218:273-9 |
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| Abstract |
GTP-cyclohydrolase I is the first enzyme in the biosynthetic pathway leading to folic acid and tetrahydrobiopterin. We determined the complete sequence of the GTP-cyclohydrolase I gene from the yeast Saccharomyces cerevisiae. The gene, which is located in the subtelomeric region of the right arm of chromosome VII, gives a major transcript of about 1000 nt and encodes a protein of 243 amino acids, which is highly homologous to the GTP-cyclohydrolase I from bacteria to man. We obtained by gene replacement a knock-out mutant that shows a recessive conditional lethality due to folinic acid auxotrophy, and lacks any detectable specific enzymatic activity. The gene was identified as FOL2, previously genetically mapped in the same region (J. Game, personal communication). |
| Links |
PubMed Online version:10.1006/bbrc.1996.0048 |
| Keywords |
Amino Acid Sequence; Animals; Bacteria/enzymology; Bacteria/genetics; Chromosome Mapping; Chromosomes, Fungal; Drosophila/genetics; GTP Cyclohydrolase/chemistry; GTP Cyclohydrolase/genetics; Genes, Fungal; Genes, Lethal; Humans; Mice; Molecular Sequence Data; Mutagenesis; Plasmids; Polymerase Chain Reaction; Rats; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Sequence Homology, Amino Acid; Telomere |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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See also
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