PMID:8544822

Citation	Ursic, D, DeMarini, DJ and Culbertson, MR (1995) Inactivation of the yeast Sen1 protein affects the localization of nucleolar proteins. Mol. Gen. Genet. 249:571-84
Abstract	A mutation in the Saccharomyces cerevisiae SEN1 gene causes accumulation of end-matured, intron-containing pre-tRNAs. Cells containing the thermosensitive sen1-1 mutation exhibit reduced tRNA splicing endonuclease activity. However, Sen1p is not the catalytic subunit of this enzyme. We have used Sen1p-specific antibodies for cell fractionation studies and immunofluorescent microscopy and determined that Sen1p is a low abundance protein of about 239 kDa. It localizes to the nucleus with a granular distribution. We verified that a region in SEN1 containing a putative nuclear localization signal sequence (NLS) is necessary for nuclear targeting. Furthermore, we found that inactivation of Sen1p by temperature shift of a strain carrying sen1-1 leads to mislocalization of two nucleolar proteins, Nop1 and Ssb1. Possible mechanisms are discussed for several related nuclear functions of Sen1p, including tRNA splicing and the maintenance of a normal crescent-shaped nucleolus.
Links	PubMed
Keywords	Amino Acid Sequence; Biological Transport; Cell Compartmentation; Cell Nucleolus/metabolism; Cell Nucleus/metabolism; DNA Helicases; Fluorescent Antibody Technique, Indirect; Fungal Proteins/genetics; Fungal Proteins/metabolism; Genes, Fungal; HSP70 Heat-Shock Proteins; Molecular Sequence Data; Mutation; Nuclear Proteins/metabolism; RNA Helicases; RNA Ligase (ATP)/deficiency; RNA Precursors/metabolism; RNA Splicing; RNA, Transfer/metabolism; Ribonucleoproteins, Small Nucleolar; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins; Sequence Deletion; Structure-Activity Relationship
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