PMID:8505303

Citation	Dong, Z, Onrust, R, Skangalis, M and O'Donnell, M (1993) DNA polymerase III accessory proteins. I. holA and holB encoding delta and delta'. J. Biol. Chem. 268:11758-65
Abstract	The genes encoding the delta and delta' subunits of the 10-subunit Escherichia coli replicase, DNA polymerase III holoenzyme, have been identified and sequenced. The holA gene encoding delta is located downstream of rlpB at 15.2 min and predicts a 38.7 kda protein. The holB gene encoding delta' is located at 24.3 min and predicts a 36.9-kDa protein. Hence the delta and delta' subunits are unrelated proteins encoded by separate genes. The genes have been used to express and purify delta and delta' in quantity. The predicted amino acid sequence of delta' is homologous to the sequences of the tau and gamma subunits revealing a large amount of structural redundancy within the holoenzyme.
Links	PubMed
Keywords	Amino Acid Sequence; Base Sequence; Chromatography, Affinity; Chromatography, Ion Exchange; Chromosome Mapping; Chromosomes, Bacterial; DNA Polymerase III/genetics; DNA Polymerase III/isolation & purification; DNA Polymerase III/metabolism; DNA Replication; DNA, Bacterial/genetics; DNA, Bacterial/isolation & purification; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Genetic Vectors; Kinetics; Macromolecular Substances; Molecular Sequence Data; Molecular Weight; Oligodeoxyribonucleotides; Open Reading Frames; Plasmids; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Restriction Mapping; Sequence Homology, Amino Acid
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ECOLX:F4M1R7		GO:0009360: DNA polymerase III complex	ECO:0000247:	PMID:8505303	C		Figure 6.	complete CACAO 4477

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