PMID:8491670

Citation	De Schepper, GG, Vander Perk, C, Westerveld, A, Oosting, J and Van Noorden, CJ (1993) In situ glucose-6-phosphate dehydrogenase activity during development of pre-implantation mouse embryos. Histochem. J. 25:299-303
Abstract	Glucose-6-phosphate dehydrogenase activity was analysed cytophotometrically in oocytes and pre-implantation embryos of mice. A bimodal distribution pattern was not found. Therefore, female and male embryos could not be discriminated on the basis of linkage of the enzyme with the X-chromosome during the pre-implantation period. The dehydrogenase activity in ovulated eggs and pre-implantation embryos up to the 8-cell stage was 65% of that present in follicular oocytes. In morulae and blastulae, the activity was further decreased to a level that was only 10-20% of the activity present in oocytes. The dramatic decrease in dehydrogenase activity could not be explained by modulation of the enzyme molecules, because KM values did not vary strongly. It is unlikely that the abundant activity of glucose-6-phosphate dehydrogenase in oocytes is due to high activity of the pentose phosphate pathway because of the low activity of 6-phosphogluconate dehydrogenase, the next step in this pathway. It is concluded that high activity of glucose-6-phosphate dehydrogenase in oocytes is needed for keeping oocytes viable, and for generation of NADPH which is important for the fertilization process.
Links	PubMed
Keywords	Animals; Blastocyst/enzymology; Embryonic and Fetal Development; Female; Formazans/chemistry; Glucosephosphate Dehydrogenase/metabolism; Male; Mice; NADP/metabolism; Oocytes/enzymology
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