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PMID:8468294

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Citation

Benson, AK and Haldenwang, WG (1993) Regulation of sigma B levels and activity in Bacillus subtilis. J. Bacteriol. 175:2347-56

Abstract

The sigB operon of Bacillus subtilis encodes sigma B plus three additional proteins (RsbV, RsbW, and RsbX) that regulate sigma B activity. Using an anti-sigma B monoclonal antibody to monitor the levels of sigma B protein, PSPAC to control the expression of the sigB operon, and a ctc-lacZ reporter system to monitor sigma B activity, we observed that the rsbV and rsbW products control sigma B activity at the ctc promoter independently of their effects on sigma B levels. In contrast, RsbX was found to have no effect on expression of ctc when the sigB operon was controlled by PSPAC. The data are consistent with RsbV and RsbW being regulators of sigma B activity and RsbX acting primarily as a negative regulator of sigB operon expression. Evidence that stationary-phase induction of the sigma B-dependent ctc promoter is accomplished by a reduction in RsbW-dependent inhibition of sigma B activity is also presented. In addition, Western blot (immunoblot) analyses of sigB operon expression demonstrated that sigma B accumulation is coupled to the synthesis of its primary inhibitor (RsbW). This finding is consistent with RsbW and sigma B being present within the cell in equivalent amounts, a circumstance that would permit RsbW to directly influence sigma B activity by a direct protein-protein interaction.

Links

PubMed PMC204523

Keywords

Animals; Antibodies, Monoclonal/immunology; Antibody Specificity; Bacillus subtilis/genetics; Bacillus subtilis/growth & development; Bacillus subtilis/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/immunology; Bacterial Proteins/metabolism; Base Sequence; Culture Media; Gene Expression Regulation, Bacterial; Genes, Bacterial; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Mutation; Operon; Sigma Factor/genetics; Sigma Factor/immunology; Sigma Factor/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

BACSU:RSBW

GO:0016989: sigma factor antagonist activity

ECO:0000315:

F

Figure 4 shows a large increase in the sigma b protein in rsbW mutants

complete
CACAO 2134

BACSU:RSBW

enables

GO:0016989: sigma factor antagonist activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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