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PMID:8455608
| Citation |
Arceci, RJ, King, AA, Simon, MC, Orkin, SH and Wilson, DB (1993) Mouse GATA-4: a retinoic acid-inducible GATA-binding transcription factor expressed in endodermally derived tissues and heart. Mol. Cell. Biol. 13:2235-46 |
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| Abstract |
We report the cDNA cloning and characterization of mouse GATA-4, a new member of the family of zinc finger transcription factors that bind a core GATA motif. GATA-4 cDNA was identified by screening a 6.5-day mouse embryo library with oligonucleotide probes corresponding to a highly conserved region of the finger domains. Like other proteins of the family, GATA-4 is approximately 50 kDa in size and contains two zinc finger domains of the form C-X-N-C-(X17)-C-N-X-C. Cotransfection assays in heterologous cells demonstrate that GATA-4 trans activates reporter constructs containing GATA promoter elements. Northern (RNA) analysis and in situ hybridization show that GATA-4 mRNA is expressed in the heart, intestinal epithelium, primitive endoderm, and gonads. Retinoic acid-induced differentiation of mouse F9 cells into visceral or parietal endoderm is accompanied by increased expression of GATA-4 mRNA and protein. In vitro differentiation of embryonic stem cells into embryoid bodies is also associated with increased GATA-4 expression. We conclude that GATA-4 is a tissue-specific, retinoic acid-inducible, and developmentally regulated transcription factor. On the basis of its tissue distribution, we speculate that GATA-4 plays a role in gene expression in the heart, intestinal epithelium, primitive endoderm, and gonads. |
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| Keywords |
Amino Acid Sequence; Animals; Base Sequence; Cell Differentiation; Cloning, Molecular; Consensus Sequence; DNA-Binding Proteins/genetics; GATA4 Transcription Factor; Gene Expression Regulation; Genes; In Situ Hybridization; Mice; Molecular Sequence Data; Promoter Regions, Genetic; RNA, Messenger/genetics; Sequence Alignment; Sequence Deletion; Structure-Activity Relationship; Transcription Factors/genetics; Transcriptional Activation; Tretinoin/pharmacology; Zinc Fingers |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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