PMID:8419289

Citation	Muthukumar, G, Suhng, SH, Magee, PT, Jewell, RD and Primerano, DA (1993) The Saccharomyces cerevisiae SPR1 gene encodes a sporulation-specific exo-1,3-beta-glucanase which contributes to ascospore thermoresistance. J. Bacteriol. 175:386-94
Abstract	A number of genes have been shown to be transcribed specifically during sporulation in Saccharomyces cerevisiae, yet their developmental function is unknown. The SPR1 gene is transcribed during only the late stages of sporulation. We have sequenced the SPR1 gene and found that it has extensive DNA and protein sequence homology to the S. cerevisiae EXG1 gene which encodes an exo-1,3-beta-glucanase expressed during vegetative growth (C. R. Vasquez de Aldana, J. Correa, P. San Segundo, A. Bueno, A. R. Nebrada, E. Mendez, and F. del Ray, Gene 97:173-182, 1991). We show that spr1 mutant cells do not hydrolyze p-nitrophenyl-beta-D-glucoside or laminarin in a whole-cell assay for exo-1,3-beta-glucanases. In addition to the absence of this enzymatic activity, spr1 mutant spores exhibit reduced thermoresistance relative to isogenic wild-type spores. These observations are consistent with the notion that SPR1 encodes a sporulation-specific exo-1,3-beta-glucanase.
Links	PubMed PMC196152
Keywords	Amino Acid Sequence; Base Sequence; Blotting, Southern; DNA, Fungal/genetics; DNA, Fungal/isolation & purification; Gene Expression Regulation, Fungal; Genes, Fungal; Glucan 1,3-beta-Glucosidase; Hot Temperature; Molecular Sequence Data; Open Reading Frames; Plasmids; Restriction Mapping; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/physiology; Sequence Homology, Amino Acid; Spores, Fungal/physiology; Transcription, Genetic; beta-Glucosidase/genetics; beta-Glucosidase/metabolism
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.