PMID:8289784

Citation	La Rosa, FA, Pierce, JW and Sonenshein, GE (1994) Differential regulation of the c-myc oncogene promoter by the NF-kappa B rel family of transcription factors. Mol. Cell. Biol. 14:1039-44
Abstract	The murine c-myc gene contains two elements responsive to the rel-oncogene-related family of NF-kappa B factors. Previously we have shown that factor binding to these two NF-kappa B elements mediates induction of transcription of the c-myc promoter upon interleukin-1 treatment of human dermal fibroblasts and human T-cell leukemia virus type I tax gene expression in T cells (D. J. Kessler, M. P. Duyao, D. B. Spicer, and G. E. Sonenshein, J. Exp. Med. 176:787-792, 1992; M. P. Duyao, D. J. Kessler, D. B. Spicer, C. Bartholomew, J. L. Cleveland, M. Siekevitz, and G. E. Sonenshein, J. Biol. Chem. 267:16288-16291, 1992). To begin to delineate the specific roles of the individual members of the NF-kappa B family, here we have tested their effects on activation of a c-myc promoter/exon 1-CAT construct in NIH 3T3 cells. Classical NF-kappa B (p65/p50) was a potent transcriptional activator of the c-myc promoter. Cotransfection with either p65 alone or p65 in combination with p50 mediated significant induction. In contrast, expression of either v-rel or chicken c-rel failed to transactivate, while murine c-rel induced c-myc promoter activity only slightly. Furthermore, induction by classical NF-kappa B was inhibited by coexpression of either v-rel or chicken c-rel. Thus, individual members of the rel family have differential effects of the c-myc promoter, which can modulate overall transcriptional activity and allow for precise regulation of this oncogene under diverse physiologic conditions.
Links	PubMed PMC358459
Keywords	3T3 Cells; Animals; Base Sequence; Chickens; Chloramphenicol O-Acetyltransferase/biosynthesis; Chloramphenicol O-Acetyltransferase/metabolism; DNA/metabolism; Gene Expression Regulation; Genes, myc; Kinetics; Mice; Molecular Sequence Data; Mutagenesis, Site-Directed; NF-kappa B/metabolism; Oligodeoxyribonucleotides/metabolism; Oncogene Proteins v-rel; Oncogenes; Promoter Regions, Genetic; Proto-Oncogene Proteins/biosynthesis; Proto-Oncogene Proteins/metabolism; Proto-Oncogene Proteins c-rel; Proto-Oncogenes; Retroviridae Proteins, Oncogenic/metabolism; Transcription Factors/metabolism; Transcription, Genetic; Transcriptional Activation; Transfection
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