PMID:8288128

Citation	Lee, J, Jongeward, GD and Sternberg, PW (1994) unc-101, a gene required for many aspects of Caenorhabditis elegans development and behavior, encodes a clathrin-associated protein. Genes Dev. 8:60-73
Abstract	Our genetic analysis indicates that the unc-101 gene of Caenorhabditis elegans is required for many aspects of development and behavior, including negative regulation of vulval differentiation. We have cloned unc-101 and found that it encodes a homolog of the mammalian medium chains of clathrin-associated protein complexes located at the trans-Golgi and the plasma membrane, AP47 and AP50, respectively. Therefore, clathrin-mediated events might contribute to the negative regulation of vulval differentiation. Comparison of sequences, including a full-length sequence of a C. elegans AP50 homolog, reveals that UNC-101 is most closely related to AP47. Mouse AP47 and nematode UNC-101 proteins are functionally equivalent as assayed in transgenic nematodes. We have sequenced the mutant alleles of unc-101 identified in various genetic screens and shown that all but one are deletions or nonsense mutations, suggesting that these alleles severely reduce unc-101 function.
Links	PubMed
Keywords	Adaptor Protein Complex 2; Adaptor Protein Complex mu Subunits; Adaptor Proteins, Vesicular Transport; Amino Acid Sequence; Animals; Base Sequence; Behavior, Animal; Caenorhabditis elegans/embryology; Caenorhabditis elegans/genetics; Caenorhabditis elegans Proteins; Clathrin/metabolism; Cloning, Molecular; DNA; Embryonic Induction; Female; Helminth Proteins/genetics; Helminth Proteins/metabolism; Mice; Molecular Sequence Data; Mutation; Nerve Tissue Proteins/physiology; Phenotype; Phosphoproteins/physiology; Sequence Homology, Amino Acid; Vulva/embryology
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Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
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