PMID:8253272

Citation	Andres, AJ, Fletcher, JC, Karim, FD and Thummel, CS (1993) Molecular analysis of the initiation of insect metamorphosis: a comparative study of Drosophila ecdysteroid-regulated transcription. Dev. Biol. 160:388-404
Abstract	More than 50 ecdysteroid-regulated Drosophila genes have been described in the literature. These genes were identified using several different ecdysteroid-responsive systems and characterized under a variety of experimental conditions. The diversity of these approaches has made it difficult to compare results and identify common responses to the hormone. As a first step toward characterizing the temporal regulation of these genes by ecdysteroids, we have examined their transcriptional activity throughout third instar larval and prepupal development using a single collection of staged animals. We see four coordinate changes in ecdysteroid-regulated gene activity during third instar larval development, at 78-88 hr, approximately 100 hr, 106-108 hr, and approximately 114 hr after egg laying. A dramatic transition in gene expression occurs at puparium formation, after which the prepupal ecdysteroid pulse induces successive waves of transcription. Our results suggest that several increases in the ecdysteroid titer during third instar larval and prepupal development program a precise temporal progression of gene activity that could direct the appropriate behavioral and developmental changes at the onset of metamorphosis.
Links	PubMed Online version:10.1006/dbio.1993.1315
Keywords	Animals; Base Sequence; Cell Line; DNA Primers; Drosophila melanogaster; Ecdysteroids; Gene Expression Regulation; Insect Hormones/physiology; Invertebrate Hormones/physiology; Larva; Metamorphosis, Biological/genetics; Molecular Sequence Data; Mutation; Steroids/physiology; Transcription, Genetic
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Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
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