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PMID:8226625
| Citation |
Lee, MH and Hatfull, GF (1993) Mycobacteriophage L5 integrase-mediated site-specific integration in vitro. J. Bacteriol. 175:6836-41 |
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| Abstract |
Mycobacteriophage L5, a temperate phage of the mycobacteria, forms stable lysogens in Mycobacterium smegmatis via site-specific integration of the phage genome. Recombination occurs within specific phage and bacterial attachment sites and is catalyzed by the phage-encoded integrase protein in vivo. We describe here the overexpression and purification of L5 integrase and its ability to mediate integrative recombination in vitro. We find that L5 integrase-mediated recombination is greatly stimulated by extracts of M. smegmatis but not by Escherichia coli extracts, purified E. coli integration host factor, or purified HU, indicating the presence of a novel mycobacterial integration host factor. |
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| Keywords |
Amino Acid Sequence; Base Sequence; DNA Nucleotidyltransferases/biosynthesis; DNA Nucleotidyltransferases/isolation & purification; DNA Nucleotidyltransferases/metabolism; DNA Primers; Integrases; Kinetics; Molecular Sequence Data; Mycobacteriophages/enzymology; Mycobacteriophages/genetics; Mycobacterium/enzymology; Mycobacterium/genetics; Plasmids; Recombinant Proteins/biosynthesis; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Recombination, Genetic; Restriction Mapping; Thermodynamics; Virus Integration |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0008979: prophage integrase activity |
ECO:0000314: |
F |
Figure 2-Shows integrative recombination mediated by Gp33. Reaction products confirm that Gp33 catalyses integrative recombination. |
complete | ||||
Notes
See also
References
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