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PMID:8164671
| Citation |
Hisamoto, N, Sugimoto, K and Matsumoto, K (1994) The Glc7 type 1 protein phosphatase of Saccharomyces cerevisiae is required for cell cycle progression in G2/M. Mol. Cell. Biol. 14:3158-65 |
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| Abstract |
We isolated a mutant carrying a conditional mutation in the GLC7 gene, encoding the catalytic subunit of a type 1 protein phosphatase, by selection of suppressors that restored the growth defect of cdc24 mutants at high temperature and simultaneously conferred cold-sensitive growth. This cold sensitivity for growth is caused by a single mutation (glc7Y-170) at position 170 of the Glc7 protein, resulting in replacement of cysteine with tyrosine. Genetic analysis suggested that the glc7Y-170 allele is associated with a recessive negative phenotype, reducing the activity of Glc7 in the cell. The glc7Y-170 mutant missegregated chromosome III at the permissive temperature, arrested growth as large-budded cells at the restrictive temperature, exhibited a significant increase in the number of nuclei at or in the neck, and had a short spindle. Furthermore, the glc7Y-170 mutant exhibited a high level of CDC28-dependent protein kinase activity when incubated at the restrictive temperature. These findings suggest that the glc7Y-170 mutation is defective in the G2/M phase of the cell cycle. Thus, type 1 protein phosphatase in Saccharomyces cerevisiae is essential for the G2/M transition. |
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| Keywords |
Amino Acid Sequence; Base Sequence; CDC28 Protein Kinase, S cerevisiae/metabolism; Cell Cycle; Chromosome Mapping; Chromosomes, Fungal; Cloning, Molecular; Fungal Proteins/biosynthesis; Fungal Proteins/genetics; Fungal Proteins/metabolism; G2 Phase; Genes, Fungal; Genetic Complementation Test; Genotype; Glycogen/metabolism; Mitosis; Molecular Sequence Data; Mutagenesis; Phenotype; Phosphoprotein Phosphatases/biosynthesis; Phosphoprotein Phosphatases/genetics; Phosphoprotein Phosphatases/metabolism; Plasmids; Protein Kinases/metabolism; Restriction Mapping; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/growth & development; Sequence Homology, Amino Acid |
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Significance
Annotations
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