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PMID:8159677
| Citation |
Peterson, CL, Dingwall, A and Scott, MP (1994) Five SWI/SNF gene products are components of a large multisubunit complex required for transcriptional enhancement. Proc. Natl. Acad. Sci. U.S.A. 91:2905-8 |
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| Abstract |
The Saccharomyces cerevisiae SWI1, SWI2 (SNF2), SWI3, SNF5, and SNF6 gene products play a crucial role in the regulation of transcription. We provide here direct biochemical evidence that all five SWI/SNF polypeptides are components of a large multisubunit complex. These five polypeptides coelute from a gel-filtration column with an apparent molecular mass of approximately 2 MDa. The five SWI/SNF polypeptides do not copurify when extracts are prepared from swi- or snf- mutants. We show that SWI/SNF polypeptides also remain associated during an affinity-chromatography step followed by gel filtration. Assembly of the SWI/SNF complex is not disrupted by a mutation in the putative APT-binding site of SWI2, although this mutation eliminates SWI2 function. |
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| Keywords |
Adenosine Triphosphatases; Adenosine Triphosphate/metabolism; Chromatography, Affinity; Chromosomal Proteins, Non-Histone; DNA-Binding Proteins/physiology; Deoxyribonucleoproteins/chemistry; Fungal Proteins/physiology; Macromolecular Substances; Nuclear Proteins/physiology; Protein Binding; Saccharomyces cerevisiae/chemistry; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins; Trans-Activators; Transcription Factors/physiology; Transcription, Genetic |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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See also
References
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