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PMID:8112310
| Citation |
Rosqvist, R, Magnusson, KE and Wolf-Watz, H (1994) Target cell contact triggers expression and polarized transfer of Yersinia YopE cytotoxin into mammalian cells. EMBO J. 13:964-72 |
|---|---|
| Abstract |
Pathogenic bacteria of the species Yersinia, including Yersinia pestis, block phagocytosis by macrophages. This process involves the YopE protein, which induces disruption of the host cell actin microfilament structure. Here, we show that the contact between the pathogen and the mammalian cell induces expression and then polarized transfer of YopE into the eukaryotic cell. While the bacteria remain at the surface of the target cell, the YopE cytotoxin is transferred through the host cell plasma membrane and YopE is only recovered within the cytosol of the target cell. The results suggest that the pathogen senses cell structures and focuses the transfer of YopE to occur solely at the interaction zone between the bacterium and the eukaryotic cell. The regulation of this process is shown to involve surface-located YopN sensor protein of the bacterium. |
| Links | |
| Keywords |
Bacterial Outer Membrane Proteins/genetics; Bacterial Outer Membrane Proteins/metabolism; Bacterial Toxins/genetics; Bacterial Toxins/metabolism; Biological Transport; Cytotoxins/genetics; Cytotoxins/metabolism; HeLa Cells; Humans; Virulence/genetics; Yersinia/metabolism; Yersinia/pathogenicity |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0044164: host cell cytosol |
ECO:0005804: |
C |
Figure 5 shows the localization of the YopE protein to the host cytosol. Indirect immunofluorescence was used to visualize this localization in HeLa cells transfected with Yersinia pseudotuberculosis (serotype III did not have YopE protein listed). Used broader evidence code because indirect immunofluorescence microscopy was not a term. |
complete | ||||
| GO:0030254: protein secretion by the type III secretion system |
ECO:0007042: |
P |
Figure 7 panels C and D show epifluorescence microscopy images of YopE localization in HeLa cells infected with the yopD mutant YPIII(pIB15) (Yersinia pseudotuberculosis serotype III). YopE was shown to not be dispersed throughout HeLa cells in YopD mutants in this imaging, indicating that YopD plays a role in the secretion of YopE. |
complete | ||||
| GO:0044164: host cell cytosol |
ECO:0005804: |
C |
Figure 5 shows the localization of the YopE protein to the host cytosol. Indirect immunofluorescence was used to visualize this localization in HeLa cells transfected with Yersinia pseudotuberculosis (serotype III did not have YopE protein listed). Used broader evidence code because indirect immunofluorescence microscopy was not a term. |
complete | ||||
Notes
See also
References
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