PMID:8083216

Citation	Poon, D, Campbell, AM, Bai, Y and Weil, PA (1994) Yeast Taf170 is encoded by MOT1 and exists in a TATA box-binding protein (TBP)-TBP-associated factor complex distinct from transcription factor IID. J. Biol. Chem. 269:23135-40
Abstract	Our characterization of the Saccharomyces cerevisiae TATA box-binding protein (TBP) has led to the identification of nine specific yeast TBP-associated factors (TAFs) ranging in size from 170 to 25 kDa. The amino acid sequence derived from a purified TAF with an apparent M(r) of 170,000 indicates that yeast Taf170 is encoded by the essential yeast gene MOT1. We describe in this report a series of experiments that demonstrate that the protein encoded by MOT1 is a bona fide yeast TAF and that Taf170 forms a separate complex with TBP distinct from the RNA polymerase II-specific multisubunit transcription factor IID TBP-TAF complex. The significance of this unique TBP-Taf170 complex regarding transcriptional regulation is discussed.
Links	PubMed
Keywords	Adenosine Triphosphatases; Amino Acid Sequence; Base Sequence; Blotting, Western; Cloning, Molecular; DNA Helicases/genetics; DNA Helicases/isolation & purification; DNA Helicases/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Electrophoresis, Polyacrylamide Gel; Molecular Sequence Data; Oligodeoxyribonucleotides; Precipitin Tests; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins; TATA Box; TATA-Binding Protein Associated Factors; TATA-Box Binding Protein; Transcription Factor TFIID; Transcription Factors/genetics; Transcription Factors/isolation & purification; Transcription Factors/metabolism
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