PMID:8045886

Citation	Barilla, D, Caramori, T and Galizzi, A (1994) Coupling of flagellin gene transcription to flagellar assembly in Bacillus subtilis. J. Bacteriol. 176:4558-64
Abstract	The regulation of flagellin gene expression in Bacillus subtilis was examined in vivo by means of a lacZ translational fusion to the flagellin structural gene (hag). We have tested the effects of two known mutations (flaA4 and flaA15) in the major flagellar operon and of three deletions. One deletion was in frame in the fliI cistron, one was out of frame in the fliK cistron, and the last spanned about 21 kb of the flaA operon. In all instances, the expression of the flagellin gene was defective. Flagellin gene expression was restored in the strain with the 21-kb deletion by overexpression of the sigD gene under control of the isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible spac promoter. These results indicate that transcription of the flagellin gene is dependent on the formation of the flagellar basal body but that such a requirement can be bypassed by overexpression of sigD. Lack of expression of hag was observed in the presence of flaD1, flaD2, and delta sin mutations as well.
Links	PubMed PMC196275
Keywords	Bacillus subtilis/cytology; Bacillus subtilis/genetics; Bacterial Proteins/genetics; Base Sequence; DNA-Binding Proteins/genetics; Flagella/physiology; Flagellin/genetics; Genes, Bacterial; Molecular Sequence Data; Operon/genetics; Proteins; Proton-Translocating ATPases; Recombinant Fusion Proteins/biosynthesis; Repressor Proteins/genetics; Sequence Deletion; Transcription, Genetic
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BACSU:FLIG		GO:0001539: ciliary or flagellar motility	ECO:0000270:		P		Figure 3D shows that wild-type strain shows motility and shows that deletion mutants are non-motile	complete CACAO 3965

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