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PMID:7969117
| Citation |
Dailey, L, Yuan, H and Basilico, C (1994) Interaction between a novel F9-specific factor and octamer-binding proteins is required for cell-type-restricted activity of the fibroblast growth factor 4 enhancer. Mol. Cell. Biol. 14:7758-69 |
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| Abstract |
Understanding how diverse transcription patterns are achieved through common factor binding elements is a fundamental question that underlies much of developmental and cellular biology. One example is provided by the fibroblast growth factor 4 (FGF-4) gene, whose expression is restricted to specific embryonic tissues during development and to undifferentiated embryonal carcinoma cells in tissue culture. Analysis of the cis- and trans-acting elements required for the activity of the previously identified FGF-4 enhancer in F9 embryonal carcinoma cells showed that enhancer function depends on sequences that bind Sp1 and ubiquitous as well as F9-specific octamer-binding proteins. However, sequences immediately upstream of the octamer motif, which conform to a binding site for the high-mobility group (HMG) domain factor family, were also critical to enhancer function. We have identified a novel F9-specific factor, Fx, which specifically recognizes this motif. Fx formed complexes with either Oct-1 or Oct-3 in a template-dependent manner. The ability of different enhancer variants to form the Oct-Fx complexes correlated with enhancer activity, indicating that these complexes play an essential role in transcriptional activation of the FGF-4 gene. Thus, while FGF-4 enhancer function is octamer site dependent, its developmentally restricted activity is determined by the interaction of octamer-binding proteins with the tissue-specific factor Fx. |
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| Keywords |
Animals; Base Sequence; Cell Line; DNA-Binding Proteins/metabolism; Enhancer Elements, Genetic; Fibroblast Growth Factor 4; Fibroblast Growth Factors/genetics; Gene Expression Regulation, Developmental; Host Cell Factor C1; Macromolecular Substances; Mice; Molecular Sequence Data; Mutagenesis, Site-Directed; Nuclear Proteins/metabolism; Octamer Transcription Factor-1; Octamer Transcription Factor-3; Proto-Oncogene Proteins/genetics; Sp1 Transcription Factor/metabolism; Structure-Activity Relationship; Transcription Factors/metabolism; Transcription, Genetic |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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