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Hilton, DJ, Hilton, AA, Raicevic, A, Rakar, S, Harrison-Smith, M, Gough, NM, Begley, CG, Metcalf, D, Nicola, NA and Willson, TA (1994) Cloning of a murine IL-11 receptor alpha-chain; requirement for gp130 for high affinity binding and signal transduction. EMBO J. 13:4765-75
An adult mouse liver cDNA library was screened with oligonucleotides corresponding to the conserved WSXWS motif of the haemopoietin receptor family. Using this method, cDNA clones encoding a novel receptor were isolated. The new receptor, named NR1, was most similar in sequence and predicted structure to the alpha-chain of the IL-6 receptor and mRNA was expressed in the 3T3-L1 pre-adipocytic cell line and in a range of primary tissues. Expression of NR1 in the factor-dependent haemopoietic cell line Ba/F3 resulted in the generation of low affinity receptors for IL-11 (Kd approximately 10 nM). The capacity to bind IL-11 with high affinity (Kd = 300-800 pM) appeared to require coexpression of both NR1 and gp130, the common subunit of the IL-6, leukaemia inhibitory factor (LIF), oncostatin M (OSM) and ciliary neurotrophic factor (CNTF) receptors. The expression of both NR1 and gp130 was also necessary for Ba/F3 cells to proliferate and M1 cells to undergo macrophage differentiation in response to IL-11.
3T3 Cells; Amino Acid Sequence; Animals; Antigens, CD; Base Sequence; Cell Division/genetics; Cell Line; Cloning, Molecular; Cytokine Receptor gp130; DNA, Complementary; Interleukin-11 Receptor alpha Subunit; Membrane Glycoproteins/genetics; Membrane Glycoproteins/metabolism; Membrane Glycoproteins/physiology; Mice; Molecular Sequence Data; RNA, Messenger/genetics; Receptors, Interleukin/chemistry; Receptors, Interleukin/metabolism; Receptors, Interleukin/physiology; Receptors, Interleukin-11; Sequence Homology, Amino Acid; Signal Transduction
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