GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:7945330
| Citation |
Rosfjord, E and Rizzino, A (1994) The octamer motif present in the Rex-1 promoter binds Oct-1 and Oct-3 expressed by EC cells and ES cells. Biochem. Biophys. Res. Commun. 203:1795-802 |
|---|---|
| Abstract |
Rex-1 is a zinc finger-containing gene that is expressed in embryonal carcinoma (EC) cells and embryonic stem (ES) cells. Upon differentiation with retinoic acid (RA), transcription of the Rex-1 gene decreases rapidly. Analysis of the 5'-flanking region of the Rex-1 gene identified a consensus motif for the octamer family of transcription factors that stimulates expression from the Rex-1 promoter. In this report, we utilized gel mobility shift analysis to examine the binding of transcription factors to the Rex-1 octamer motif. F9 EC cells, D3 ES cells, and human NT2/D1 EC cells each from at least two prominent DNA/protein complexes with the octamer motif. Supershift analysis identifies Oct-1 and Oct-3 in these complexes. When F9 EC cells are induced to differentiate by treatment with RA for 48 h, there is a complete loss of the DNA/protein complex containing Oct-3. In contrast, the other DNA/protein complexes, including the DNA/protein complex containing Oct-1, can still be detected. These results provide support for a role of Oct-3 in the transcription of the Rex-1 gene. |
| Links |
PubMed Online version:10.1006/bbrc.1994.2395 |
| Keywords |
Animals; Base Sequence; Carcinoma, Embryonal/metabolism; Cell Differentiation/physiology; Cell Line; Cell Nucleus/metabolism; DNA-Binding Proteins/biosynthesis; DNA-Binding Proteins/metabolism; Embryo, Mammalian; Gene Expression; Genes, pX; Host Cell Factor C1; Mice; Molecular Sequence Data; Nuclear Proteins/isolation & purification; Nuclear Proteins/metabolism; Octamer Transcription Factor-1; Octamer Transcription Factor-3; Promoter Regions, Genetic; Stem Cells/cytology; Stem Cells/drug effects; Stem Cells/metabolism; Transcription Factors/biosynthesis; Transcription Factors/metabolism; Transcription, Genetic; Tretinoin/pharmacology; Tumor Cells, Cultured |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
