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PMID:7929329
| Citation |
Paul, MF, Ackerman, S, Yue, J, Arselin, G, Velours, J, Tzagolof, A and Ackermann S [corrected to Ackerman, S (1994) Cloning of the yeast ATP3 gene coding for the gamma-subunit of F1 and characterization of atp3 mutants. J. Biol. Chem. 269:26158-64 |
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| Abstract |
Saccharomyces cerevisiae pet mutants, of complementation group G115, are deficient in mitochondrial ATPase and have properties indicative of defective F1. In this study we show that C287/LU1, a mutant belonging to group G115, is complemented by the yeast nuclear ATP3 gene coding for the gamma-subunit of the mitochondrial F1-ATPase. The amino-terminal sequence of the mature gamma-subunit matches the sequence encoded by ATP3 starting with the 34th amino acid confirming the identity of the gene, and earlier evidence indicating that this F1 component is synthesized as a precursor with a long amino-terminal extension. The properties of the mitochondrial ATPase have been studied in C287/LU1 with an Ala273-->Val substitution in the carboxyl-terminal region of the gamma-subunit and in W303 delta ATP3, a mutant lacking the gamma-subunit as a result of a deletion in ATP3. Both strains have negligible ATPase activity but near normal concentrations of the alpha- and beta-subunits of F1. In W303 delta ATP3, the subunits do not form a stable F1 oligomer nor are they firmly associated with F0. This is not true of C287/LU1, which was found to assemble an F1-F0 complex. These data indicate that the yeast gamma-subunit has dual functions, one in catalysis of ATP hydrolysis/synthesis and the second in assembly/stability of F1. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Cloning, Molecular; Genes, Fungal; Molecular Sequence Data; Mutation; Proton-Translocating ATPases/chemistry; Proton-Translocating ATPases/genetics; Proton-Translocating ATPases/physiology; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Structure-Activity Relationship |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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