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PMID:7923404
| Citation |
Bidard, F, Blondin, B, Dequin, S, Vezinhet, F and Barre, P (1994) Cloning and analysis of a FLO5 flocculation gene from S. cerevisiae. Curr. Genet. 25:196-201 |
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| Abstract |
A yeast flocculation gene was isolated from a genomic library of an FLO5 strain of S. cerevisiae on the basis of its ability to trigger flocculation in a non-flocculent strain. Characterization of the cloned gene by restriction mapping, Southern analysis, and chromosome mapping have shown that it corresponds to a FLO5 gene previously located on chromosome I and that this gene is related to the already described FLO1 gene. A study of gene expression in different yeast strains has indicated that, while this gene is dominant, its expression can be suppressed in some genetic backgrounds. A Northern-blot analysis has demonstrated that the same 5000-nt transcript was present in an FLO5 and an FLO1 strain. A gene disruption experiment has led to the conclusion that another flocculation gene is present and can be active in the FLO5 strain we used. |
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| Keywords |
Chromosome Mapping; Chromosomes, Fungal; Cloning, Molecular; DNA, Fungal/genetics; Gene Expression Regulation, Fungal; Genes, Fungal; Phenotype; Saccharomyces cerevisiae/genetics; Transcription, Genetic |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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See also
References
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