PMID:7868621

Citation	Ogawa, K, Akagawa, E, Yamane, K, Sun, ZW, LaCelle, M, Zuber, P and Nakano, MM (1995) The nasB operon and nasA gene are required for nitrate/nitrite assimilation in Bacillus subtilis. J. Bacteriol. 177:1409-13
Abstract	Bacillus subtilis can use either nitrate or nitrite as a sole source of nitrogen. The isolation of the nasABCDEF genes of B. subtilis, which are required for nitrate/nitrite assimilation, is reported. The probable gene products include subunits of nitrate/nitrite reductases and an enzyme involved in the synthesis of siroheme, a cofactor for nitrite reductase.
Links	PubMed PMC176753
Keywords	Anion Transport Proteins; Bacillus subtilis/genetics; Bacillus subtilis/metabolism; Bacterial Proteins/genetics; Carrier Proteins/genetics; Chromosome Walking; Genes, Bacterial/genetics; Nitrate Reductase; Nitrate Reductases/genetics; Nitrates/metabolism; Nitrites/metabolism; Open Reading Frames/genetics; Operon/genetics; Sequence Analysis, DNA; Sequence Homology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BACSU:NASA		GO:0015706: nitrate transport	ECO:0000315:		P		"The nasA mutant is defective in nitrate assimilation (Table 3) but possesses high nitrate reductase activity (Table 4)," suggesting that nasA may act as a nitrate transport.	complete
BACSU:NASA		GO:0015706: nitrate transport	ECO:0000250:	UniProtKB:P10903	P		Similarity over a short region between nasA and narK, the E. coli nitrate transporter, has been detected, indicating that nasA may act as a nitrate transporter.	complete
BACSU:NASB		GO:0008940: nitrate reductase activity	ECO:0000315:		F		Table 4 supports that nasB encodes the subunit for nitrate reductase, as the mutant deletion strain showed very low nitrate reductase activity.	complete

References

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