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PMID:7860592

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Citation

Glaser, P, Danchin, A, Kunst, F, Zuber, P and Nakano, MM (1995) Identification and isolation of a gene required for nitrate assimilation and anaerobic growth of Bacillus subtilis. J. Bacteriol. 177:1112-5

Abstract

The Bacillus subtilis narA locus was shown to include narQ and narA. The putative product of narQ is similar to FdhD, which is required for formate dehydrogenase activity in Escherichia coli. NarA showed homology to MoaA, a protein involved in biosynthesis of the molybdenum cofactor for nitrate reductase and formate dehydrogenase. Analysis of mutants showed that narA but not narQ is required for both nitrate assimilation and respiration.

Links

PubMed PMC176711

Keywords

Anaerobiosis/genetics; Bacillus subtilis/genetics; Bacillus subtilis/growth & development; Bacterial Proteins/genetics; Cell Division/genetics; Coenzymes; Escherichia coli Proteins; Formate Dehydrogenases/analysis; Genes, Bacterial/genetics; Genetic Complementation Test; Genomic Library; Membrane Proteins/genetics; Metalloproteins; Molecular Sequence Data; Nitrate Reductase; Nitrate Reductases/analysis; Nitrates/metabolism; Phosphoproteins/genetics; Pteridines; Restriction Mapping; Transformation, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

BACSU:MOAA

GO:0042128: nitrate assimilation

ECO:0000315:

P

Table 3: narA is required for nitrate assimilation.

complete

BACSU:MOAA

GO:0009061:

ECO:0000315:

Fgure 2: narA is required for anaerobic respiration.

complete

BACSU:MOAA

GO:0008863: formate dehydrogenase activity

ECO:0000316:

UniProtKB:P39756


F

Table 4: Both narA and narQ are required for formate dehydrogenase activity.

complete


See also

References

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