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PMID:7860592
Citation |
Glaser, P, Danchin, A, Kunst, F, Zuber, P and Nakano, MM (1995) Identification and isolation of a gene required for nitrate assimilation and anaerobic growth of Bacillus subtilis. J. Bacteriol. 177:1112-5 |
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Abstract |
The Bacillus subtilis narA locus was shown to include narQ and narA. The putative product of narQ is similar to FdhD, which is required for formate dehydrogenase activity in Escherichia coli. NarA showed homology to MoaA, a protein involved in biosynthesis of the molybdenum cofactor for nitrate reductase and formate dehydrogenase. Analysis of mutants showed that narA but not narQ is required for both nitrate assimilation and respiration. |
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Keywords |
Anaerobiosis/genetics; Bacillus subtilis/genetics; Bacillus subtilis/growth & development; Bacterial Proteins/genetics; Cell Division/genetics; Coenzymes; Escherichia coli Proteins; Formate Dehydrogenases/analysis; Genes, Bacterial/genetics; Genetic Complementation Test; Genomic Library; Membrane Proteins/genetics; Metalloproteins; Molecular Sequence Data; Nitrate Reductase; Nitrate Reductases/analysis; Nitrates/metabolism; Phosphoproteins/genetics; Pteridines; Restriction Mapping; Transformation, Genetic |
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Significance
Annotations
Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0042128: nitrate assimilation |
ECO:0000315: |
P |
Table 3: narA is required for nitrate assimilation. |
complete | ||||
GO:0009061: |
ECO:0000315: |
Fgure 2: narA is required for anaerobic respiration. |
complete | |||||
GO:0008863: formate dehydrogenase activity |
ECO:0000316: |
UniProtKB:P39756
|
F |
Table 4: Both narA and narQ are required for formate dehydrogenase activity. |
complete | |||
See also
References
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