GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:7753822
| Citation |
Helaakoski, T, Annunen, P, Vuori, K, MacNeil, IA, Pihlajaniemi, T and Kivirikko, KI (1995) Cloning, baculovirus expression, and characterization of a second mouse prolyl 4-hydroxylase alpha-subunit isoform: formation of an alpha 2 beta 2 tetramer with the protein disulfide-isomerase/beta subunit. Proc. Natl. Acad. Sci. U.S.A. 92:4427-31 |
|---|---|
| Abstract |
Prolyl 4-hydroxylase (EC 1.14.11.2) catalyzes the posttranslational formation of 4-hydroxyproline in collagens. The vertebrate enzyme is an alpha 2 beta 2 tetramer, the beta subunit of which is a highly unusual multifunctional polypeptide, being identical to protein disulfide-isomerase (EC 5.3.4.1). We report here the cloning of a second mouse alpha subunit isoform, termed the alpha (II) subunit. This polypeptide consists of 518 aa and a signal peptide of 19 aa. The processed polypeptide is one residue longer than the mouse alpha (I) subunit (the previously known type), the cloning of which is also reported here. The overall amino acid sequence identity between the mouse alpha (II) and alpha (I) subunits is 63%. The mRNA for the alpha (II) subunit was found to be expressed in a variety of mouse tissues. When the alpha (II) subunit was expressed together with the human protein disulfide-isomerase/beta subunit in insect cells by baculovirus vectors, an active prolyl 4-hydroxylase was formed, and this protein appeared to be an alpha (II) 2 beta 2 tetramer. The activity of this enzyme was very similar to that of the human alpha (I) 2 beta 2 tetramer, and most of its catalytic properties were also highly similar, but it differed distinctly from the latter in that it was inhibited by poly(L-proline) only at very high concentrations. This property may explain why the type II enzyme was not recognized earlier, as an early step in the standard purification procedure for prolyl 4-hydroxylase is affinity chromatography on a poly(L-proline) column. |
| Links | |
| Keywords |
Amino Acid Sequence; Animals; Baculoviridae; Base Sequence; Blotting, Northern; Brain/enzymology; Caenorhabditis elegans/enzymology; Cell Line; Cloning, Molecular; DNA Primers; DNA, Complementary; Gene Expression; Humans; Isoenzymes/biosynthesis; Isoenzymes/chemistry; Isoenzymes/metabolism; Isomerases/biosynthesis; Isomerases/chemistry; Kinetics; Macromolecular Substances; Male; Mice; Molecular Sequence Data; Organ Specificity; Procollagen-Proline Dioxygenase/biosynthesis; Procollagen-Proline Dioxygenase/chemistry; Procollagen-Proline Dioxygenase/metabolism; Protein Disulfide-Isomerases; RNA, Messenger/analysis; RNA, Messenger/biosynthesis; Recombinant Proteins/biosynthesis; Recombinant Proteins/chemistry; Recombinant Proteins/metabolism; Sequence Homology, Amino Acid; Spodoptera; Substrate Specificity |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
