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PMID:7750142

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Citation

Benito-Moreno, RM, Miaczynska, M, Bauer, BE, Schweyen, RJ and Ragnini, A (1994) Mrs6p, the yeast homologue of the mammalian choroideraemia protein: immunological evidence for its function as the Ypt1p Rab escort protein. Curr. Genet. 27:23-5

Abstract

The Saccharomyces cerevisiae MRS6 gene belongs to the same gene family as that responsible for the mammalian Rab escort protein (REP) and the Rab GDP dissociation inhibitor protein (GDI). Both REP and GDI are regulators of the Ras-related small G-proteins Rab/YPT1 which are involved in intracellular vesicular trafficking in yeast and in mammals. Here we characterize an antiserum directed against Mrs6p and show that it specifically inhibits the geranylation of the YPT1 protein in an in vitro assay. These findings provide direct evidence for the role of Mrs6p as the REP component of the yeast Rab geranylgeranyl transferase enzyme.

Links

PubMed

Keywords

Adaptor Proteins, Signal Transducing; Alkyl and Aryl Transferases; Animals; Base Sequence; Carrier Proteins/antagonists & inhibitors; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cloning, Molecular; Fungal Proteins/antagonists & inhibitors; Fungal Proteins/genetics; Fungal Proteins/metabolism; GTP-Binding Proteins/metabolism; Genes, Fungal; Humans; Immune Sera/pharmacology; Molecular Sequence Data; Protein Prenylation; Protein Processing, Post-Translational; Rabbits; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/immunology; Saccharomyces cerevisiae Proteins; Species Specificity; Transferases/metabolism; rab GTP-Binding Proteins

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:RAEP

involved_in

GO:0090630: activation of GTPase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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