GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:7729555
| Citation |
Bufe, A, Schramm, G, Keown, MB, Schlaak, M and Becker, WM (1995) Major allergen Phl p Vb in timothy grass is a novel pollen RNase. FEBS Lett. 363:6-12 |
|---|---|
| Abstract |
A cDNA coding for the major group V allergen Phl p Vb was isolated from a timothy grass pollen cDNA library by immunoscreening with a specific monoclonal antibody. It was discovered for the first time that the recombinant Phl p Vb pollen allergen after expression and purification has ribonuclease activity. High homology of Phl p Vb to other group V allergens in grass pollen indicates similar function. By RNase activity gel of natural pollen extract of timothy grass and consecutive Western blot analysis of the excised proteins, the RNase active bands were shown to be group V allergens. Additionally it was demonstrated that an homologous protein to Phl p Vb in the mother plant could be induced by salicylic acid. This indicates that group Vb allergens may be involved in host-pathogen interactions because in pollen they are quickly exported RNases and in the mother plant they depend on a hormone which is related to expression of plant resistance genes. |
| Links | |
| Keywords |
Allergens; Amino Acid Sequence; Antibodies, Monoclonal; Base Sequence; Blotting, Western; Chromatography, High Pressure Liquid; DNA, Complementary/chemistry; DNA, Complementary/isolation & purification; Electrophoresis, Polyacrylamide Gel; Molecular Sequence Data; Plant Proteins/chemistry; Plant Proteins/genetics; Plant Proteins/metabolism; Pollen; RNA, Messenger/metabolism; Recombinant Proteins/chemistry; Recombinant Proteins/metabolism; Ribonucleases/metabolism; Salicylates/pharmacology; Salicylic Acid; Sequence Homology |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0004540: ribonuclease activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0004540: ribonuclease activity |
ECO:0000314: |
F |
Fig 3 shows mRNA is degraded by purified Phl p Vb |
complete | ||||
See also
References
See Help:References for how to manage references in GONUTS.
