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PMID:7665600
| Citation |
Jiang, Y, Proteau, P, Poulter, D and Ferro-Novick, S (1995) BTS1 encodes a geranylgeranyl diphosphate synthase in Saccharomyces cerevisiae. J. Biol. Chem. 270:21793-9 |
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| Abstract |
Protein prenylation utilizes different types of isoprenoids groups, namely farnesyl and geranylgeranyl, to modify proteins. These lipophilic moieties attach to carboxyl-terminal cysteine residues to promote the association of soluble proteins to membranes. Most prenylated proteins are geranylgeranylated. Geranylgeranylation is catalyzed by two different prenyltransferases, the type I and type II geranylgeranyl transferases, both of which utilize geranylgeranyl diphosphate as a lipid donor. In the yeast Saccharomyces cerevisiae, the BET2 gene encodes the beta-subunit of the type II geranylgeranyl transferase. Mutations in this gene cause a defect in the geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic. In an attempt to analyze those genes whose products may interact with Bet2, we isolated a suppressor of the bet2-1 mutant. This suppressor gene, called BTS1, encodes the yeast geranylgeranyl diphosphate synthase. BTS1 is not essential for the vegetative growth of cells; however, disrupting it impedes the geranylgeranylation of many cellular proteins and renders cells cold sensitive for growth. Our findings imply that BTS1 suppresses the bet2-1 mutant by increasing the intracellular pool of geranylgeranyl diphosphate. |
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| Keywords |
Alkyl and Aryl Transferases; Amino Acid Sequence; Base Sequence; Cloning, Molecular; Conserved Sequence; DNA, Fungal/genetics; DNA, Fungal/isolation & purification; Escherichia coli; Gene Expression; Genes, Fungal; Genomic Library; Geranylgeranyl-Diphosphate Geranylgeranyltransferase; Kinetics; Molecular Sequence Data; Mutagenesis; Oxidoreductases/biosynthesis; Oxidoreductases/genetics; Oxidoreductases/metabolism; Recombinant Proteins/biosynthesis; Recombinant Proteins/metabolism; Restriction Mapping; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/growth & development; Sequence Homology, Amino Acid; Suppression, Genetic |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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