PMID:7608095

Citation	Hagman, KE and Shafer, WM (1995) Transcriptional control of the mtr efflux system of Neisseria gonorrhoeae. J. Bacteriol. 177:4162-5
Abstract	The capacity of Neisseria gonorrhoeae to resist structurally diverse hydrophobic agents (HAs) because of the mtr (multiple transferable resistance) efflux system was found to be regulated at the level of transcription by two distinct mechanisms. This was surmised because a deletion that removed > 90% of the coding sequence of the mtrR (multiple transferrable resistance regulator) gene or a single-base-pair deletion within a 13-bp inverted repeat sequence located in its promoter resulted in altered expression of the mtrC gene; mtrC encodes a 44-kDa membrane lipoprotein essential for the efflux of HAs. However, the single-base-pair deletion had the more significant impact on gene expression since it resulted in the loss of expression of mtrR and a threefold increase in the expression of mtrC. Hence, the mtr efflux system in gonococci is subject to both MtrR-dependent and MtrR-independent regulation, and the levels of mtrC mRNA correlate well with HA resistance levels in gonococci.
Links	PubMed PMC177154
Keywords	Bacterial Proteins; Base Sequence; Biological Transport/genetics; Carrier Proteins/genetics; Drug Resistance, Microbial/genetics; Ferredoxin-NADP Reductase; Gene Expression Regulation, Bacterial; Lipoproteins/genetics; Membrane Proteins/genetics; Membrane Transport Proteins; Molecular Sequence Data; Mutation; Neisseria gonorrhoeae/genetics; Neisseria gonorrhoeae/metabolism; RNA, Messenger/analysis; Repressor Proteins/genetics; Transcription, Genetic
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
NEIGO:MTRR		GO:0006355: regulation of transcription, DNA-dependent	ECO:0000315:		P		Table 1. shows results from mutant stains. Figure 3 shows mRNA level from mutant strains.	complete

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