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PMID:7545952
| Citation |
Kelly, SL and Sim, E (1994) Arylamine N-acetyltransferase in Balb/c mice: identification of a novel mouse isoenzyme by cloning and expression in vitro. Biochem. J. 302 ( Pt 2):347-53 |
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| Abstract |
Three genes encoding arylamine N-acetyltransferase were identified in Balb/c mice. All three genes were cloned from genomic DNA, sequenced and expressed in a bacterial expression system. Two of the genes corresponded to Nat-1 and Nat-2 which have been previously identified in A/J and C57B1/6 strains of mice (Martell et al., 1991). The new gene, designated Nat-3, can be distinguished from the other mouse Nat genes both by specific amplification using PCR and by restriction-endonuclease digestion. The products of all three genes are demonstrated to catalyse acetylation of aminofluorene and anisidine following expression in Escherichia coli. |
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| Keywords |
Acetylation; Amino Acid Sequence; Animals; Arylamine N-Acetyltransferase/chemistry; Arylamine N-Acetyltransferase/genetics; Arylamine N-Acetyltransferase/metabolism; Base Sequence; Blotting, Southern; Cloning, Molecular; DNA/chemistry; DNA/genetics; Electrophoresis, Polyacrylamide Gel; Escherichia coli/genetics; Isoenzymes/chemistry; Isoenzymes/genetics; Isoenzymes/metabolism; Mice; Mice, Inbred A; Mice, Inbred BALB C; Mice, Inbred C57BL; Molecular Sequence Data; Polymerase Chain Reaction; Recombinant Proteins/biosynthesis; Recombinant Proteins/metabolism; Restriction Mapping |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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See also
References
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