PMID:7514104

Citation	Shiota, J, Nishimura, H, Okamoto, H, Yu, B, Hattori, S, Abe, M, Okada, T, Nozawa, S, Tsurui, H and Hirose, S (1994) A unique murine CD43 epitope Lp-3: distinct distribution from another CD43 epitope S7. Cell. Immunol. 155:402-13
Abstract	In foregoing studies, we found a unique B cell differentiation antigen Lp-3 which is expressed on pre-B and premature B cells in the bone marrow, but is negative on bone marrow mature B cells and peripheral resting B cells. Nonetheless, Lp-3 was clearly positive on the majority of CD5 B(B1) cells. When we examined the biochemical nature and partial amino acid sequences of purified 132-kDa Lp-3 molecules and the nucleotide sequence of the cDNA clones, we found that Lp-3 is an epitope of CD43. Thus, the monoclonal antibody (mAb) Lp-3 may be the first mAb to murine CD43 defined by primary target structure analysis. Comparison of tissue distribution of Lp-3 and S7, an epitope previously suggested to associate with murine CD43, showed that they were similarly distributed on thymocytes, peripheral B and T cells, granulocytes, and platelets. In the bone marrow, while both Lp-3 and S7 were negative on mature B cells, the former was positive on all B lineage cells at an early ontogeny and the latter was positive only on the minor population of pre-B cells and pro-B cells. Lp-3 and S7 epitopes also showed different distributions on basement membranes of renal glomerulus, bronchus, and endometrium, lining cells of choroid plexus and muscular cells of arterioles in a variety of tissues. As CD43 has various isoforms generated by different degrees of glycosylation of the common core peptide, it is likely that Lp-3 and S7 are associated with different CD43 isoforms.
Links	PubMed
Keywords	Amino Acid Sequence; Animals; Antibodies, Monoclonal; Antigens, CD; Antigens, CD43; Base Sequence; Cell Differentiation; Epitopes/genetics; Epitopes/immunology; Flow Cytometry; Fluorescent Antibody Technique; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Peptide Fragments/chemistry; Sequence Analysis; Sialoglycoproteins/genetics; Sialoglycoproteins/immunology; Tissue Distribution; Tumor Cells, Cultured
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