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PMID:7472454
| Citation |
Weinshenker, D, Garriga, G and Thomas, JH (1995) Genetic and pharmacological analysis of neurotransmitters controlling egg laying in C. elegans. J. Neurosci. 15:6975-85 |
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| Abstract |
We have investigated the neurotransmitters used to control egg-laying in C. elegans. Previous studies suggested that 5-HT released by the HSN motor neurons stimulates egg laying, and that tricyclic antidepressants potentiate egg laying by blocking reuptake of 5-HT by the HSN neurons. We report studies of the wild type and a mutant that lacks detectable 5-HT that suggest 5-HT is not required for egg-laying. Furthermore, we find that ACh is required for egg laying in response to 5-HT, suggesting that 5-HT is not sufficient to activate egg laying. The dominant egl-2(n693) mutation, which causes animals to lay eggs in response to tricyclics but not 5-HT, also conflicts with the model for egg laying. Experiments in which the HSN neurons or 5-HT are removed from egl-2 animals indicate that the action of tricyclics cannot be explained by a block of 5-HT reuptake. We find that D2 family dopamine antagonists can also induce egg laying in egl-2(n693) mutants, and that dopamine inhibits egg laying in the wild type. These results suggest that dominant egl-2 mutations activate an inhibitory dopaminergic pathway that can be blocked by tricyclics and D2 antagonists. We also find that these drugs stimulate egg laying in mutants lacking 5-HT or the HSN neurons, consistent with a target on the egg-laying muscles. In contrast to tricyclics, fluoxetine and other selective 5-HT reuptake inhibitors appear to be specific for 5-HT reuptake in C. elegans egg laying. |
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| Keywords |
Acetylcholine/physiology; Animals; Antidepressive Agents, Tricyclic/pharmacology; Caenorhabditis elegans/physiology; Dopamine Antagonists/pharmacology; Female; Fluoxetine/pharmacology; Genetic Techniques; Intestines/innervation; Muscle, Smooth/innervation; Mutation; Neural Inhibition; Neurons/physiology; Neurotransmitter Agents/physiology; Oviposition/drug effects; Oviposition/physiology; Serotonin/genetics; Serotonin/physiology |
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