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PMID:6992959
| Citation |
Waygood, EB and Steeves, T (1980) Enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli. Purification to homogeneity and some properties. Can. J. Biochem. 58:40-8 |
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| Abstract |
Enzyme I of the phosphoenolpyruvate - sugar phosphotransferase system (PTS) has been purified to homogeneity from Escherichia coli. A merodiploid strain P650 which had an extra copy of the gene for enzyme I resulting in a twofold increase in the amount of activity was used. The enzyme is a dimer of 67 000 +/- 5000 molecular weight subunits. At low protein concentration and 4 degrees C the monomer predominates, while at room temperature the dimer predominates. At higher protein concentrations (2 to 10 mg) this reversible temperature-dependent association-dissociation is not found. Enzyme I has a pH optimum of pH 7.2, a Km for HPr of 9 +/- 3 microM, a Km for phosphoenolpyruvate of 0.18 +/- 0.04 mM, and kinetics that are consistent with a bi bi Ping-Pong mechanism. No allosteric regulation of kinetic activity has been found. The amino acid composition has been determined and the epsilon 1% 280 nm is 4.4. Evidence suggests that the phosphorylated form of enzyme I is more stable. |
| Links | |
| Keywords |
Cell Division; Escherichia coli/enzymology; Histidine/analysis; Hydrogen-Ion Concentration; Kinetics; Models, Chemical; Molecular Weight; Phosphoenolpyruvate Sugar Phosphotransferase System/isolation & purification; Phosphoenolpyruvate Sugar Phosphotransferase System/metabolism; Protein Conformation; Temperature |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0008965: phosphoenolpyruvate-protein phosphotransferase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0009401: phosphoenolpyruvate-dependent sugar phosphotransferase system |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0019197: phosphoenolpyruvate-dependent sugar phosphotransferase complex |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
See also
References
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