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PMID:6778869

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Citation

Shuman, HA and Silhavy, TJ (1981) Identification of the malK gene product. A peripheral membrane component of the Escherichia coli maltose transport system. J. Biol. Chem. 256:560-2

Abstract

The malK gene product of Escherichia coli has been identified through the use of a previously described technique that employs gene fusions (Shuman, H. A., Silhavy, T. J., and Beckwith, J. R. (1980) J. Biol. Chem. 255, 168-174). This protein, along with the four other products of the malB locus, comprise the complete maltose transport system. The malK protein has a molecular weight of approximately 40,000 and is located in the cell envelope. In mutant strains which lack another component of the transport system, the malG protein, the malK protein is located in the cytoplasm. This alteration in location suggests that the malK protein is associated with the inner surface of the cytoplasmic membrane via an interaction with the malG protein.

Links

PubMed

Keywords

ATP-Binding Cassette Transporters; Bacterial Proteins; Carrier Proteins/genetics; Cell Membrane/metabolism; Chromosomes, Bacterial/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Genes; Genotype; Maltose/genetics; Maltose-Binding Proteins; Molecular Weight; Monosaccharide Transport Proteins; Mutation; beta-Galactosidase/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:MALK

part_of

GO:0031234: extrinsic component of cytoplasmic side of plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

Notes

See also

References

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