GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:6735465
| Citation |
Selsted, ME, Szklarek, D and Lehrer, RI (1984) Purification and antibacterial activity of antimicrobial peptides of rabbit granulocytes. Infect. Immun. 45:150-4 |
|---|---|
| Abstract |
Six antimicrobial peptides, corresponding to the family of "lysosomal cationic proteins" described previously by Zeya and Spitznagel (H. I. Zeya and J. K. Spitznagel, J. Bacteriol. 91:750-754, 1966; H. I. Zeya and J. K. Spitznagel, J. Bacteriol. 91:755-762, 1966), were purified from rabbit peritoneal granulocytes by preparative acrylamide gel electrophoresis and reversed-phase high-pressure liquid chromatography. Each of the peptides was of low molecular weight (ca. 4,000) as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The two most cationic peptides, NP-1 and NP-2, were active against a broad spectrum of gram-positive and gram-negative bacteria. The remaining four peptides, NP-3A, NP-3B, NP-4, and NP-5, had more selective antibacterial activity. None of the peptides was active against Bordetella bronchiseptica, a common pathogen of domestic rabbits. Antibacterial activity was best expressed at near neutral pH under conditions of low ionic strength. |
| Links | |
| Keywords |
Animals; Bacteria/drug effects; Blood Bactericidal Activity; Blood Proteins/isolation & purification; Blood Proteins/toxicity; Chromatography, High Pressure Liquid; Granulocytes/physiology; Hydrogen-Ion Concentration; Microbial Sensitivity Tests; Osmolar Concentration; Rabbits; Species Specificity |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0050829: defense response to Gram-negative bacterium |
ECO:0000314: |
P |
table 1 |
complete | ||||
| GO:0050830: defense response to Gram-positive bacterium |
ECO:0000314: |
P |
table 1 |
complete | ||||
| GO:0001909: leukocyte mediated cytotoxicity |
ECO:0000314: |
P |
Table 1 and methods |
complete | ||||
| GO:0042581: specific granule |
ECO:0000314: |
C |
methods |
complete | ||||
| GO:0050830: defense response to Gram-positive bacterium |
ECO:0000314: |
P |
table 1 |
complete | ||||
| GO:0050829: defense response to Gram-negative bacterium |
ECO:0000314: |
P |
table 1 |
complete | ||||
| GO:0042581: specific granule |
ECO:0000314: |
C |
METHODS |
complete | ||||
| GO:0001909: leukocyte mediated cytotoxicity |
ECO:0000314: |
P |
Table 1 and METHODS |
complete | ||||
| GO:0050830: defense response to Gram-positive bacterium |
ECO:0000314: |
P |
table 1 |
complete | ||||
| GO:0050829: defense response to Gram-negative bacterium |
ECO:0000314: |
P |
table 1 |
complete | ||||
| GO:0042581: specific granule |
ECO:0000314: |
C |
methods |
complete | ||||
| GO:0001909: leukocyte mediated cytotoxicity |
ECO:0000314: |
P |
Table 1 and methods |
complete | ||||
| GO:0050829: defense response to Gram-negative bacterium |
ECO:0000314: |
P |
table 1 |
complete | ||||
| GO:0050830: defense response to Gram-positive bacterium |
ECO:0000314: |
P |
table 1 |
complete | ||||
| GO:0042581: specific granule |
ECO:0000314: |
C |
METHODS |
complete | ||||
| GO:0001909: leukocyte mediated cytotoxicity |
ECO:0000314: |
P |
Table 1 and METHODS |
complete | ||||
| GO:0050829: |
ECO:0000314: |
table 1 |
complete | |||||
| GO:0050830: defense response to Gram-positive bacterium |
ECO:0000314: |
P |
table 1 |
complete | ||||
| GO:0042581: specific granule |
ECO:0000314: |
C |
methods |
complete | ||||
See also
References
See Help:References for how to manage references in GONUTS.
