PMID:6444532

Citation	Vora, S, Corash, L, Engel, WK, Durham, S, Seaman, C and Piomelli, S (1980) The molecular mechanism of the inherited phosphofructokinase deficiency associated with hemolysis and myopathy. Blood 55:629-35
Abstract	Normal human erythrocyte phosphofructokinase (ATP: D-fructose-6, P-1-phosphotransferase, EC 2.7.1.11; PFK) has recently been shown to consist of a heterogeneous mixture of five tetrameric isozymes: M4, M3L, M2L2, ML3, and L4 (M, muscle type; L, liver type). In the light of these findings, we have investigated the molecular basis of the inherited erythrocyte PFK deficiency associated with myopathy and hemolysis (Tarui disease). The propositus, a 31-yr-old male, suffered from muscle weakness and myoglobinuria on exertion. He showed mild erythrocytosis despite laboratory evidence of hemolysis. In his erythrocytes a metabolic crossover point was found at the level of PFK; 2,3-diphosphoglycerate (2,3-DPG) was also significantly reduced. The PFK from the patient's erythrocytes consisted exclusively of the L4 isozyme, and there was a complete absence of the other four. The leukocyte and platelet PFKs from the patient showed normal activities, chromatographic profiles, and precipitation with anti-M4 antibody. These studies provide direct evidence that in Tarui disease the M-type subunits are absent; but the liver- and platelet-type subunits of PFK are unaffected. The paradox of mild erythrocytosis despite hemolysis reflects the decreased production of 2,3-DPG.
Links	PubMed
Keywords	Adult; Blood Platelets/enzymology; Diphosphoglyceric Acids/deficiency; Erythrocytes/enzymology; Hemolysis; Humans; Isoenzymes/analysis; Leukocytes/enzymology; Liver/enzymology; Male; Muscle Hypotonia/genetics; Myoglobinuria/genetics; Phosphofructokinase-1/deficiency
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