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PMID:6434524
| Citation |
Kawamura, F and Doi, RH (1984) Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases. J. Bacteriol. 160:442-4 |
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| Abstract |
A mutant strain of Bacillus subtilis carrying lesions in the structural genes for extracellular neutral (nprE) and serine (aprA) proteases was constructed by the gene conversion technique. This mutant had less than 4% of the extracellular protease activity of the wild type and sporulated normally, indicating that neither of these sporulation-associated proteases is essential for development. |
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| Keywords |
Bacillus subtilis/enzymology; Bacillus subtilis/genetics; Chromosome Deletion; Endopeptidases/genetics; Genes; Genes, Bacterial; Genotype; Mutation; Neprilysin; Serine Endopeptidases; Species Specificity; Spores, Bacterial/physiology |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0006508: proteolysis |
ECO:0000315: |
P |
This mutant (nprE/aprA) had less than 4% of the extracellular protease activity of the wild type... Table 2. |
complete | ||||
| GO:0006508: proteolysis |
ECO:0000315: |
P |
Only 2 to 4% of the protease activity of the wild type was found in the supernatants from cultures of DB104 and DB105 (both aprA-). Table 2. |
complete | ||||
See also
References
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