PMID:6377310

Citation	Engebrecht, J and Silverman, M (1984) Identification of genes and gene products necessary for bacterial bioluminescence. Proc. Natl. Acad. Sci. U.S.A. 81:4154-8
Abstract	Expression of luminescence in Escherichia coli was recently achieved by cloning genes from the marine bacterium Vibrio fischeri. One DNA fragment on a hybrid plasmid encoded regulatory functions and enzymatic activities necessary for light production. We report the results of a genetic analysis to identify the luminescence genes (lux) that reside on this recombinant plasmid. lux gene mutations were generated by hydroxylamine treatment, and these mutations were ordered on a linear map by complementation in trans with a series of polar transposon insertions on other plasmids. lux genes were defined by complementation of lux gene defects on pairs of plasmids in trans in E. coli. Hybrid plasmids were also used to direct the synthesis of polypeptides in the E. coli minicell system. Seven lux genes and the corresponding gene products were identified from the complementation analysis and the minicell programing experiments. These genes, in the order of their position on a linear map, and the apparent molecular weights of the gene products are luxR (27,000), luxI (25,000), luxC (53,000), luxD (33,000), luxA (40,000), luxB (38,000), and luxE (42,000). From the luminescence phenotypes of E. coli containing mutant plasmids, functions were assigned to these genes: luxA, luxB, luxC, luxD, and luxE encode enzymes for light production and luxR and luxI encode regulatory functions.
Links	PubMed PMC345387
Keywords	Cloning, Molecular; Escherichia coli/genetics; Genes, Bacterial; Genetic Complementation Test; Luminescent Measurements; Mutation; Vibrio/genetics
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
VIBFI:LUXC	involved_in	GO:0008218: bioluminescence	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ALIFS:LUXE		GO:0008218: bioluminescence	ECO:0000315:		P		In figure 1, complementing pairs of hybrid plasmids with lux mutations produced light, but non-complimenting pairs harbored little to no light.	complete CACAO 8713
ALIFS:LUXE	involved_in	GO:0008218: bioluminescence	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ALIFS:LUXC		GO:0008218: bioluminescence	ECO:0000315:		P		In figure 1, complementing pairs of hybrid plasmids with lux mutations produced light, but non-complimenting pairs harbored little to no light.	complete CACAO 8715
ALIFS:LUXC	involved_in	GO:0008218: bioluminescence	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ALIFS:Q9S3Z1		GO:0008218: bioluminescence	ECO:0000315:		P		In figure 1, complementing pairs of hybrid plasmids with lux mutations produced light, but non-complimenting pairs harbored little to no light.	complete CACAO 8712
ALIFS:Q9S3Z1	involved_in	GO:0008218: bioluminescence	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ALIFS:LUXD		GO:0008218: bioluminescence	ECO:0000315:		P		In figure 1, complementing pairs of hybrid plasmids with lux mutations produced light, but non-complimenting pairs harbored little to no light.	complete CACAO 8714
ALIFS:LUXD	involved_in	GO:0008218: bioluminescence	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ALIFS:LUXB		GO:0008218: bioluminescence	ECO:0000315:		P		In figure 1, complementing pairs of hybrid plasmids with lux mutations produced light, but non-complimenting pairs harbored little to no light.	complete CACAO 8716
ALIFS:LUXB	involved_in	GO:0008218: bioluminescence	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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