GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:6352051
Citation |
Miller, DM 3rd, Ortiz, I, Berliner, GC and Epstein, HF (1983) Differential localization of two myosins within nematode thick filaments. Cell 34:477-90 |
---|---|
Abstract |
The body wall muscle cells of the nematode, Caenorhabditis elegans, contain two unique types of myosin heavy chain, A and B. We have utilized an immunochemical approach to define the structural location of these two myosins within body wall muscle thick filaments. By immunofluorescence microscopy, myosin B antibodies label the thick filament-containing A-bands of body wall muscle with the exception of a thin gap at the center of each A-band, and myosin A antibodies react to form a medial fluorescent stripe within each A-band. The complexes of these monoclonal antibodies with isolated thick filaments were negatively stained and studied by electron microscopy. The myosin B antibody reacts with the polar regions of all filaments but does not react with a central 0.9 micron zone. The myosin A antibody reacts with a central 1.8 micron zone in all filaments but does not react with the polar regions. |
Links | |
Keywords |
Animals; Antibodies, Monoclonal; Caenorhabditis; Fluorescent Antibody Technique; Microscopy, Electron; Muscles/analysis; Myosins/analysis; Myosins/immunology; Tissue Distribution |
edit table |
Significance
Annotations
Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
edit table |
See also
References
See Help:References for how to manage references in GONUTS.