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PMID:6296074
| Citation |
Kanaya, S and Crouch, RJ (1983) DNA sequence of the gene coding for Escherichia coli ribonuclease H. J. Biol. Chem. 258:1276-81 |
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| Abstract |
The gene for Escherichia coli ribonuclease H has been studied by use of a plasmid which contains a segment of the E. coli chromosome. The genomic DNA was subcloned from pLC28-22 to pBR322 by use of various restriction enzymes. Such subcloning limited the RNase H gene to a piece of DNA no longer than 760 base pairs. Cells bearing plasmids containing the RNase H gene produce as much as 10-15 times the normal amount of RNase H without any drastic effect on maintenance of the plasmid or cell growth. DNA sequence analysis has permitted the prediction of a protein whose molecular weight is 17,559 (155 amino acid residues). The predicted sequence was confirmed by amino acid analysis, NH2-terminal amino acid sequence, and size determination of highly purified RNase H. |
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| Keywords |
Amino Acids/analysis; Base Sequence; DNA, Bacterial/analysis; Endoribonucleases/analysis; Endoribonucleases/genetics; Escherichia coli/enzymology; Molecular Weight; Plasmids; Ribonuclease H |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
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See also
References
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