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PMID:6223276
| Citation |
Macreadie, IG, Novitski, CE, Maxwell, RJ, John, U, Ooi, BG, McMullen, GL, Lukins, HB, Linnane, AW and Nagley, P (1983) Biogenesis of mitochondria: the mitochondrial gene (aap1) coding for mitochondrial ATPase subunit 8 in Saccharomyces cerevisiae. Nucleic Acids Res. 11:4435-51 |
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| Abstract |
A mitochondrial gene (denoted aap1) in Saccharomyces cerevisiae has been characterized by nucleotide sequence analysis of a region of mtDNA between the oxi3 and oli2 genes. The reading frame of the aap1 gene specifies a hydrophobic polypeptide containing 48 amino acids. The functional nature of this reading frame was established by sequence analysis of a series of mit- mutants and revertants. Evidence is presented that the aap1 gene codes for a mitochondrially synthesized polypeptide associated with the mitochondrial ATPase complex. This polypeptide (denoted subunit 8) is a proteolipid whose size has been previously assumed to be 10 kilodaltons based on its mobility on SDS-polyacrylamide gels, but the sequence of the aap1 gene predicts a molecular weight of 5,815 for this protein. |
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| Keywords |
Adenosine Triphosphatases/genetics; Amino Acid Sequence; Base Sequence; DNA, Mitochondrial/genetics; Genes; Mitochondria/enzymology; Molecular Weight; Mutation; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
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See also
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