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Moore, KH, Bonema, JD and Solomon, FJ (1984) Long-chain acyl-CoA and acylcarnitine hydrolase activities in normal and ischemic rabbit heart. J. Mol. Cell. Cardiol. 16:905-13
Long-chain acyl-CoA and acylcarnitine hydrolase activities were determined in fresh and perfused rabbit heart and correlated with tissue levels of their respective substrates, long-chain acyl-CoA and acylcarnitine. In fresh heart homogenate acyl-CoA hydrolase activity was 3-fold greater than acylcarnitine hydrolase activity; sonication of homogenate doubled acyl-CoA hydrolase activity but did not significantly change acylcarnitine hydrolase activity. Hearts perfused with 10 mM glucose by the nonrecirculating Langendorff method had depressed levels of acyl-CoA hydrolase activity under both aerobic and ischemic conditions. Extract from buffer-perfused heart showed increased acylcarnitine hydrolase activity and elevated levels of acylcarnitine. Homogenate acyl-CoA hydrolase activity was not sedimented by centrifugal forces up to 50,000 X g; however, less than 25% of homogenate acylcarnitine hydrolase activity remained in the 50,000 X g supernatant. The hypolipidemic drug clofibrate was an effective in vitro inhibitor of acylcarnitine hydrolase activity but not of acyl-CoA hydrolase activity. The fatty acid analog tetradecylglycidic acid inhibited only acyl-CoA hydrolase activity. These results suggest that acyl-CoA hydrolase and acylcarnitine hydrolase activities are differentially affected in the perfused heart by substrate levels and oxygen availability. In addition, the diverse response of these two hydrolase activities to a variety of biochemical parameters implies that the observed hydrolyses of palmitoyl-CoA and palmitoylcarnitine are catalyzed by at least two separate hydrolase enzymes.
Animals; Carboxylic Ester Hydrolases/analysis; Carboxylic Ester Hydrolases/antagonists & inhibitors; Clofibrate/pharmacology; Coronary Disease/enzymology; Epoxy Compounds/pharmacology; Fatty Acids/pharmacology; Male; Mice; Myocardium/enzymology; Palmitoyl-CoA Hydrolase/analysis; Palmitoyl-CoA Hydrolase/antagonists & inhibitors; Rabbits; Rats; Species Specificity; Thiolester Hydrolases/analysis
|Gene product||Qualifier||GO Term||Evidence Code||with/from||Aspect||Extension||Notes||Status|
|GO:0047619: acylcarnitine hydrolase activity||
This paper performs an experiment to provide necessary information about acylcarnitine hydrolase activities and function.
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