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PMID:3881388
| Citation |
Rodríguez-Tébar, A, Barbas, JA and Vázquez, D (1985) Location of some proteins involved in peptidoglycan synthesis and cell division in the inner and outer membranes of Escherichia coli. J. Bacteriol. 161:243-8 |
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| Abstract |
Inner and outer membranes of Escherichia coli were separated by isopycnic centrifugation in sucrose gradients and analyzed for the presence of penicillin-binding proteins. All penicillin-binding proteins--except penicillin-binding protein 3, which is found almost exclusively in the cytoplasmic membrane and is involved in septum formation--are also found in gradient fractions corresponding to the outer membrane. Our results support the hypothesis that approximately half of the total amount of penicillin-binding proteins may be sacculus-located proteins linked to the outer membrane, probably through peptidoglycan bridges. |
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| Keywords |
Anti-Bacterial Agents/pharmacology; Aztreonam; Bacterial Outer Membrane Proteins/analysis; Bacterial Proteins; Carboxypeptidases/analysis; Carrier Proteins/analysis; Cell Division; Cell Fractionation; Cell Membrane/analysis; Centrifugation, Density Gradient; Escherichia coli/analysis; Escherichia coli/drug effects; Escherichia coli Proteins; Hexosyltransferases; Microscopy, Electron; Muramoylpentapeptide Carboxypeptidase/analysis; Penicillin-Binding Proteins; Peptidoglycan/biosynthesis; Peptidoglycan Glycosyltransferase; Peptidyl Transferases |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
part_of |
GO:0031226: intrinsic component of plasma membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
See also
References
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