GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:3794643
| Citation |
Scott, NW and Dow, CS (1986) Purification and partial characterization of DNA-dependent RNA polymerase from Rhodomicrobium vannielii. J. Gen. Microbiol. 132:1939-49 |
|---|---|
| Abstract |
DNA-dependent RNA polymerase has been isolated from Rhodomicrobium vannielii. Like those from other eubacteria, the enzyme contained four subunits: beta and beta prime (Mr 155,000), alpha (Mr 38,000), and sigma (Mr 98,000). Analysis by isoelectric focussing showed that both alpha and sigma had several forms with different isoelectric pH values. The enzyme was sensitive to rifampicin (5 ng rifampicin ml-1 gave 50% inhibition) and capable of specific promoter selection with DNA from R. vannielii, calf thymus and phage T7D111. |
| Links | |
| Keywords |
Chromatography, Gel; Chromatography, Ion Exchange; DNA, Bacterial; DNA-Directed RNA Polymerases/genetics; DNA-Directed RNA Polymerases/isolation & purification; Electrophoresis, Polyacrylamide Gel; Isoelectric Focusing; Macromolecular Substances; Molecular Weight; Rhodospirillaceae/enzymology; Rhodospirillaceae/genetics; Templates, Genetic; Transcription, Genetic |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0003899: DNA-directed RNA polymerase activity |
IDA: Inferred from Direct Assay: |
F |
Table 1 |
complete |
| |||
See also
References
See Help:References for how to manage references in GONUTS.
